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Scientists develop new system to boost prime editing in dicot plants
A new gene-editing system could make it easier for researchers to introduce precise genetic changes into crops such as tomatoes, soybeans and rapeseed.
Summary
A new gene-editing system could make it easier for researchers to introduce precise genetic changes into crops such as tomatoes, soybeans and rapeseed. Developed by researchers in China, SmartPE improves prime editing efficiency in several dicot plants, addressing a major limitation of the technique. The new system introduces mismatches into the reverse transcriptase templates (RTTs) of two prime editing guide RNAs (pegRNAs), combining this strategy with a cis-regulatory element and an engineered reverse transcriptase.
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