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Fluorescence timing identifies eight proteins in one cell with a single staining step
An international collaboration led by the University Medical Center Göttingen (UMG), Germany, has developed a labeling method that distinguishes fluorescent labels not by color but by how long it takes a dye to emit fluorescence.
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An international team led by the University Medical Center Göttingen has created a labeling technique that separates fluorescent dyes by their emission time rather than color. The method allows eight distinct proteins to be visualized in a single staining step using standard fluorescence microscopes. The study, published in ACS Nano, demonstrates that time‑resolved fluorescence can overcome the usual limit of three or four color channels in conventional microscopy. This approach expands the capacity for simultaneous protein imaging in biomedical research.
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